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細胞增殖、活性、毒性評估指南

本指南提供相關資訊針對研究需求選擇最適合您的實驗方法,進行細胞增殖(proliferation)、活性(viability)與毒性(cytotoxicity)的評估。

細胞增殖 (Cell Proliferation)

直接計數測量細胞數
測量DNA 含量以揭示細胞分裂和 DNA 複製

方法

  • 使用細胞計數器進行計數
  • 使用螢光染劑進行DNA定量

細胞活性 (Cell Proliferation)

測量細胞健康相關參數,以評估細胞活性

方法

  • 使用MTT、WST、XTT等,測量細胞代謝活性的證據,如esterase activity, ATP, NADPH dehydrogenase activity, Mitochondria activity等。

細胞毒性 (Cell Cytotoxicity)

測量細胞死亡指標以評估細胞毒性

方法

  • 測量受損及死亡細胞滲出的LDH含量
  • 應用membrane-impermeable dyes(非膜穿透性染劑),選擇性標記膜受損細胞。

Learn more about cell analysis
(“Reagent Selection Guide, Cell Proliferation / Cell Viability / Cytotoxicity ”)


細胞增殖/毒性測試相關試劑

MTT 和 WST 技術是評估細胞增殖和細胞毒性的廣泛使用的方法。它們因其各自程序的簡單性、安全性和結果的可重複性而受到重視。WST(水溶性四唑)由 DOJINDO Laboratories 在 1990 年代開發,現在在全球範圍內用於細胞增殖測定。Cell Counting Kit-8 (CCK-8) 是一種商業產品,它利用 WST 方法提高了靈敏度。Cell Counting Kit-8是 WST 的原創產品,已在全球發表超過 5,000 多篇論文。

Product name 
MTT  Cell Counting Kit-8

ATP Assay Kit

Cell Counting Kit-F
Calcein-AM   
Target

Mitochondrial dehydrogenase activity
Cellular dehydrogenase activity
ATP
Esterase activity
Instrument

Microplate reader (colorimetric)  Microplate reader (colorimetric)
Microplate reader (luminometric)
Microplate reader, FCM, Fluorescence microscope (fluorometric)
Advantage

Less expensive
Simple procedure
High stable reagent
High sensitivity
Short reaction time(15 min)
Simple procedure
Applicable for various instruments
Short reaction time(15 min)
Disadvantage

Affected by reducing agents
Requires dissolution of reagent after reaction
Affected by reducing agents
Expensive
Serum increases background
Leakage of reagent
Product code
 M009  CK04 

- CK06 (kit) C396 (ready-to-use solution)
C326 (powder)

細胞毒性測定試劑比較

Cytotoxicity LDH Assay Kit-WST是用於評估細胞毒性的試劑盒。該測定法測量從受損細胞膜釋放的乳酸脫氫酶(LDH)。Cytotoxicity LDH Assay Kit-WST非常適合多樣本分析,可快速方便評估細胞毒性。

Product name 
Cytotoxicity LDH Assay Kit-WST
Cell Counting Kit-F
(Calcein-AM)
Propidium iodide (PI)
Trypan Blue
Target

Released Lacate dehydrogenase (LDH)
A marker (Calcein-AM) released into the supernatant (Calcein-Release Assay)
Cytoplasm(Live cell membranes are impermeable to PI, but not dead cell membranes)
Cytoplasm; cells taking up trypan blue are considered non-viable
Instrument

Microplate reader (colorimetric)  Microplate reader, FCM, Fluorescence microscope (Fluorometric)
Microplateplate reader, FCM, (fluorometric)
Microscope (colorimetric)
Advantage

・Simple procedure
・Suitable for multi-sample assay
・Convenient for cytotoxicity assay using 2 kinds of cell samples
・Less expensive
・Simple procedure
・Less expensive
・No special instruments needed
Disadvantage

・Increased background by serum
・Leakage of reagent from living cells
・Not applicable for multi-samlple assay
・False-positive by cytotoxicity
・Not applicable for multi-samples assay
Product code
CK12 CK06 (kit)
C396 (ready-to-use solution)
C326 (powder)
P378 (ready-to-use solution)
P346 (powder)
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以多個參數進行細胞毒性評估

為了驗證細胞毒性測定結果的準確性,可根據多種不同的原理測量單個樣品。Cell Counting Kit-8(代碼:CK04)和Cytotoxicity Assay Kit-WST(代碼:CK12)分別測量脫氫酶活性和 LDH 釋放量,以反應活細胞和死細胞表現。根據這些結果,隨著毒物濃度的增加,活細胞數量減少,而死細胞數量增加。