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Accutase and Accumax

Accutase and Accumax

Accutase :取代Trypsin,可溫和地分離黏附性細胞,細胞不受酵素影響,不破壞抗原表面;Accumax :Accumax兼具 protease, collagenolytic, 及 DNase 的活性,作用後細胞存活率高,應用於各種實驗方法
Accutase
產品說明:
取代Trypsin,可溫和地分離黏附性細胞,細胞不受酵素影響,不破壞抗原表面,作用後細胞存活率高且不須沖洗或添加其他物質中和,Accutase兼具 protease 及 collagenolytic 的活性。產品不含哺乳動物及微生物物質。
產品優點:
  • 可完全取代trypsin
  • 溫和且有效率地分離附著型的細胞(adherent cell line)
  • 應用在胚胎幹細胞及神經幹細胞(embryonic and neuronal stem cells)的效果特別好
  • 對細胞表面抗原的傷害最小,適合應用在後續Flow cytometry分析
  • 使用後不需要沖洗或添加其他物質中和
 
產品應用:
hESC culturing, analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, apoptosis, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.

Cell Detachment

Human MG63 Fibrosarcoma cells cultured in DMEM + 10% FBS were treated with Accutase. Treatment resulted in rapid cell detachment, a single cell suspension, and high viability. Accutase is gentle on cells; viability was 97 ± 3% even after 45 minutes in Accutase.


Accumax
產品說明:
讓細胞團塊在幾分鐘內溫和地使細胞分散,容易取得單一懸浮細胞,Accumax兼具 protease, collagenolytic, 及 DNase 的活性,作用後細胞存活率高,應用於各種實驗方法, 如 cell counting, viral transfection assays, cell sorting, and flow cytometry 以及 bioreactor scale-up。產品不含哺乳動物及微生物物質。
 
產品優點:

  • 可溫和分離組織團塊
  • 增加細胞計數的準確性
  • 可解離neuronal及prostate 細胞團塊
  • 可溫和地將細胞從3-D matrixes分離
  • 可溫和地將細胞從hollow fiber cell reactors分離
  • 可延長clumpy cell在sorting的時間,讓細胞不容易再聚集

產品應用:
hESC culturings, analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogenetransfection, neural crest cell migration assays, cell proliferation, apoptosis, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry. Accumax is used to digest primary tissue, create single cell suspensions for cell counts, and declump cells for magnetic or flow cytometry cell sorting and removing from artificial growing matrixes.
 
 
Increase in Cell Counts

Various constructs of genetically engineered CHO cells, BHK cells, and a hybridoma were grown in suspension in serum-free or protein-free medium. 
Representative cell aliquots were treated with an equal volume of PBS or ACCUMAX and incubated for 5 minutes at 37C. Cell number was then determined with a Coulter Counter.




 
Products Cata # Quantity
Accutase AT-104 100ml
Accutase AT-104-500 500ml
Accumax AM-105 100ml
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