專題產品
細胞增殖、活性、毒性評估指南
本指南提供相關資訊針對研究需求選擇最適合您的實驗方法，進行細胞增殖(proliferation)、活性(viability)與毒性(cytotoxicity)的評估。
細胞增殖 (Cell Proliferation)
直接計數測量細胞數
測量DNA 含量以揭示細胞分裂和 DNA 複製
方法
- 使用細胞計數器進行計數
- 使用螢光染劑進行DNA定量
細胞活性 (Cell Proliferation)
測量細胞健康相關參數，以評估細胞活性
方法
- 使用MTT、WST、XTT等，測量細胞代謝活性的證據，如esterase activity, ATP, NADPH dehydrogenase activity, Mitochondria activity等。
細胞毒性 (Cell Cytotoxicity)
測量細胞死亡指標以評估細胞毒性
方法
- 測量受損及死亡細胞滲出的LDH含量
- 應用membrane-impermeable dyes(非膜穿透性染劑)，選擇性標記膜受損細胞。
Learn more about cell analysis
(“Reagent Selection Guide, Cell Proliferation / Cell Viability / Cytotoxicity ”)
細胞增殖/毒性測試相關試劑
MTT 和 WST 技術是評估細胞增殖和細胞毒性的廣泛使用的方法。它們因其各自程序的簡單性、安全性和結果的可重複性而受到重視。WST（水溶性四唑）由 DOJINDO Laboratories 在 1990 年代開發，現在在全球範圍內用於細胞增殖測定。Cell Counting Kit-8 (CCK-8) 是一種商業產品，它利用 WST 方法提高了靈敏度。Cell Counting Kit-8是 WST 的原創產品，已在全球發表超過 5,000 多篇論文。
| Product name |
MTT | Cell Counting Kit-8 |
ATP Assay Kit |
Cell Counting Kit-F Calcein-AM |
| Target |
Mitochondrial dehydrogenase activity |
Cellular dehydrogenase activity |
ATP |
Esterase activity |
| Instrument |
Microplate reader (colorimetric) | Microplate reader (colorimetric) |
Microplate reader (luminometric) |
Microplate reader, FCM, Fluorescence microscope (fluorometric) |
| Advantage |
Less expensive |
Simple procedure High stable reagent |
High sensitivity Short reaction time(15 min) Simple procedure |
Applicable for various instruments Short reaction time(15 min) |
| Disadvantage |
Affected by reducing agents Requires dissolution of reagent after reaction |
Affected by reducing agents |
Expensive |
Serum increases background Leakage of reagent |
| Product code |
M009 | CK04 |
- | CK06 (kit)
C396 (ready-to-use solution) C326 (powder) |
細胞毒性測定試劑比較
Cytotoxicity LDH Assay Kit-WST是用於評估細胞毒性的試劑盒。該測定法測量從受損細胞膜釋放的乳酸脫氫酶(LDH)。Cytotoxicity LDH Assay Kit-WST非常適合多樣本分析，可快速方便評估細胞毒性。
| Product name |
Cytotoxicity LDH Assay Kit-WST |
Cell Counting Kit-F (Calcein-AM) |
Propidium iodide (PI) |
Trypan Blue |
| Target |
Released Lacate dehydrogenase (LDH) |
A marker (Calcein-AM) released into the supernatant (Calcein-Release Assay) |
Cytoplasm(Live cell membranes are impermeable to PI, but not dead cell membranes) |
Cytoplasm; cells taking up trypan blue are considered non-viable |
| Instrument |
Microplate reader (colorimetric) | Microplate reader, FCM, Fluorescence microscope (Fluorometric) |
Microplateplate reader, FCM, (fluorometric) |
Microscope (colorimetric) |
| Advantage |
・Simple procedure ・Suitable for multi-sample assay |
・Convenient for cytotoxicity assay using 2 kinds of cell samples |
・Less expensive ・Simple procedure |
・Less expensive ・No special instruments needed |
| Disadvantage |
・Increased background by serum |
・Leakage of reagent from living cells |
・Not applicable for multi-samlple assay |
・False-positive by cytotoxicity ・Not applicable for multi-samples assay |
| Product code |
CK12 | CK06 (kit) C396 (ready-to-use solution) C326 (powder) |
P378 (ready-to-use solution) P346 (powder) |
- |
以多個參數進行細胞毒性評估
為了驗證細胞毒性測定結果的準確性，可根據多種不同的原理測量單個樣品。Cell Counting Kit-8（代碼：CK04）和Cytotoxicity Assay Kit-WST（代碼：CK12）分別測量脫氫酶活性和 LDH 釋放量，以反應活細胞和死細胞表現。根據這些結果，隨著毒物濃度的增加，活細胞數量減少，而死細胞數量增加。
