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Exosome 細胞外泌體研究

Exosome

Extracellular Vesicles (EVs)

細胞外囊泡 (EV) 是由細胞分泌到細胞外的脂質結合囊泡，依大小及不同的蛋白質特徵分為三種類型

  • Apoptotic bodies (50-5000 nm) 包含完整的細胞器、染色質
  • Microvesicle (100-1000 nm)
  • Exosome (30-150 nm)

Exosome

  • 參與及應用
    • Cell–cell communication
    • Cell maintenance
    • Tumor progression
    • Biomarkers for diseases
    • Drug delivery systems
  • Where ?
    • 血漿、血清、尿液、精液、唾液、支氣管液、腦脊髓液 (CSF)、母乳、羊水、滑液、淚液、淋巴液、膽汁和胃酸

Exosome Related Product

  • Exosome Isolation /Purification
    • Ultrafiltration
    • Size-exclusion chromatography
    • Precipitation
    • Immunoaffinity-based approaches
    • Magnetic bead-based ion exchange
    • Charge-based Precipitation
    • Isolate exosome from plants 
  • 特定應用Kit 
    • Capture Beads for Flow Detection
    • Capture Beads for Elisa Detection
  • Exosome Standards 
  • Other Exosomes Related Product 
    • Exosome Membrane Permeabilization
    • Exosome Blocking
    • Exosome Tracking/ Staining Kit
    • Human Exosomal Antibody Markers
    • Exosomal miRNA Extraction 
    • Co-culture system
    • Exosome Free FBS

Exosome Isolation /Purification

Ultrafiltration

以過濾法直接分離Exosome

ExoIsolator Exosome Isolation Kit

 產品資訊

productcatalog numberpackage
ExoIsolator Exosome Isolation Kit
EX10-10
Holder ×1・Isolation Filter ×3
ExoIsolator Isolation FilterEX11-10Filter ×10

ExoFilterTM

coming soon

Size-exclusion chromatography

以純化管柱分離Exosome

Exosome Isolation Columns (SEC)

 product list產品資訊

productcatalog numberpackage
Exosome Size Exclusion Columns (Evs SEC 35 nm)SEC3512-4
純化管柱 Size: 35-350nm-4 pack
SEC3512-8純化管柱 Size: 35-350nm-8 pack
Exosome Size Exclusion Columns (Evs SEC 70 nm)
SEC7012-4純化管柱 Size: 70-1000nm-4 pack
SEC7012-8純化管柱 Size: 70-1000nm-8 pack

Precipitation

以沉澱試劑搭配離心分離Exosome

Exosome Precipitation Solutions

 product list產品資訊

productcatalog numberpackage
Exosome Precipitation Solution (Cell Culture Media and Urine)EPSTEP12 ml
Exosome Precipitation Solution (Plasma and Serum)EPStep-PS5 ml
Exosome precipitation (from Plasma + Trombin)
EPStep-T5 ml

ExoEZ Exosome Isolation Products

 產品資訊

productcatalog numberpackage
Exosome Isolation Kit for Cell CultureExoCC5050 ml
Exosome Isolation Kit for Urine
ExoUT5050 ml
Exosome Isolation Kit for Breast Milk
ExoBM5050 ml

Immunoaffinity-based approaches

以免疫結合方式分離Exosome

MagCapture™ / PS Capture™

原廠產品資訊

productcatalog number
MagCapture™ Exosome Isolation Kit PS Ver.2294-84101 290-84103
PS Capture™ Exosome Isolation Resin Kit (for large volume sample) 290-80301

Magnetic bead-based ion exchange

帶正電磁珠吸引帶負電Exosome 並分離

ExoCAS-2TM

產品資訊

ProductExoCAS-2 Mini Kit (20)ExoCAS-2 Maxi Kit (50)ExoCAS-2 CCM050 KitExoCAS-2 CCM250 Kit
catalog number70200705007105071250
test20 preps50 preps50 preps250 preps
SamplePlasma, Serum, Urine, SalivaCulture Media
sample volume20mL50mL50mL250mL
Recommended sample volume per test* 1 ml recommend Users can adjust the volume ratio (0.2 - 10 mL ) * 1 ml recommend Users can adjust the volume ratio (1 - 20 mL )
ReagentsMagnetic Bead Solution, Elution Buffer

Charge-based Precipitation

帶正電離子試劑與帶負電Exosome結合沉澱並分離

ExoPASTM

產品資訊

ProductExoPAS OriginalExoPAS CCMExoPAS AdvancedExoPAS CCM Advanced
catalog number72050720517206072061
test50 preps50 preps
samplePlasma, Serum, Urine, SalivaCulture MediaPlasma, Serum, Urine, SalivaCulture Media
sample volume0.2 - 10mL1 - 20mL0.2 - 10mL1 - 20mL
ReagentsPrecipitation Solution, Elution Buffer (Optional) Washing Buffer Precipitation Solution 1&2, Elution Buffer (Optional) Washing Buffer

Isolate exosome from plants

從植物樣品中分離Exosome

ExoPLANT-Lo / ExoPLANT-Hi

 產品資訊

productcatalog
sample
EXOSOME ISOLATION FROM HIGH WATER CONTENT PLANT MATERIAL KITExoPLANT-HiFor 200 ml plant juice
EXOSOME ISOLATION FROM LOW WATER CONTENT PLANT MATERIAL KIT ExoPLANT-LoFor 20 g plant material

特定應用Kit

Capture Beads for Flow Detection

PS Capture™ Exosome Flow Cytometry Kit

 原廠產品資訊

productcatalog numberapplication
PS Capture™ Exosome Flow Cytometry Kit
297-79701Flow, WB

應用於Flow

 product list產品資訊

productcatalog numbertest
Human CD9 Capture Beads for Flow Detection
9CB-2525 tests
Human CD63 Capture Beads for Flow Detection
63CB-25
Human CD81 Capture Beads for Flow Detection
81CB-25
Human CD274 Capture Beads for Flow Detection
274CB-25
Human CD326 (EpCAM) Capture Beads for Flow Detection
326CB-25
Human IgG1 Capture Beads for Flow Detection (Isotype Control)
IGG1CB-25
Human IgG2a Capture Beads for Flow Detection (Isotype Control)
ICIGG2ACB-25
Mouse CD63 Capture Beads for Flow Detection
MO-63CB-25

Capture Beads for Elisa Detection

PS Capture™ Exosome Flow Cytometry Kit

 原廠產品資訊

productcatalog numbertest
PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD)
297-7920196 tests
PS Capture™ Exosome ELISA Kit (Streptavidin HRP)
298-80601
CD9-Capture Human Exosome ELISA Kit (Streptavidin HRP)
296-83701
CD63-Capture Human Exosome ELISA Kit (Streptavidin HRP)
290-83601
CD81-Capture Human Exosome ELISA Kit (Streptavidin HRP)
292-83801

Exosome Standards

Lyophilized Exosome Standards 外泌體標準品

 product list產品資訊

品名貨號Unit
Lyophilized Exosome A575 cell line Standards
ExoA575100 ug
Lyophilized Exosome from RPMICell line Standards
ExoRPMI
Lyophilized Exosome from HT29 cell line Standards
ExoHT29
Lyophilized Exosome from MCF7 cell Standards
ExoMCF7
Lyophilized Exosome from PC3 cell line Standards
ExoPC3
Lyophilized Exosome from SERUM Standards
ExoSERUM
Adipose-derived Mesenchymal stem/stromal cells (MSCs) derived exosomes
ExoMSC
Exosomes from CaCo2, StandardsExoCaCo2
Exosomes from PANC-1, Standards
ExoPANC1

Ready-to-use Exosomes 外泌體標準品

產品資訊

Cat. No.SpeciesCell LinesDisease Patterns
EAA01HumanHuman Retinal Microvascular Endothelial CellNormal
EAA02Human Retinal Pericyte
EAA04Human Brain Pericyte
EAA05Human Heart Myocyte
EAA06Human Lung Fibroblast
EAA07Human Hair Follicle Dermal Papila cell
EAA08Human Dermal Fibroblast
EAA09Human Periodontal Ligament Cell
EAA10Human Colon Fibroblast
EAA12Human Heart smooth muscle Cell
EAA13Human Lung smooth muscle cell
EAA14Human Skin Keratinocyte
EAA15Human Gingival Keratinocyte
EAA16Human Kidney Epithelial Cell
EAA18Human Uterine Fibroblast
EAA19Human Uterine smooth muscle cell
EAA20Human Mammary Epithelial cell
EAA25Human T Cell (CD8+)
EAB01Human Lung CarcinomaLung cancer / Carninoma
EAB02Human Peripheral blood Burkitts lymphomaBurkitts Lymphoma
EAB03Human Colon AdenocarcinomaColon cancer / Adenocarcinoma
EAB04Human Pancreas CarcinomaPancreas cancer / Carcinoma
EAB05Human Kidney AdenocarcinomaKidney cancer / Adenocarcinoma
EAB06Human Skin Malignant MelanomaSkin Malignant melanoma
EAB07Human Liver Hepatocellular CarcinomaLiver cancer / Carcinoma
EAB12Human COPD Bronchial/Tracheal Epithelial CellLung / COPD
EAB15Human Skin Aneuploidy KeratinocyteSkin Aneuploidy
EAB16Human Cervix AdenocarcinomaCervix cancer / Adenocarcinoma
EAC01Human Adipose Mesenchymal Stem CellStem Cell
EAC02Human Bone Marrrow Mesenchymal Stem Cell
EAC03Human Umbilical Cord Mesenchymal Stem Cell
EBA01Canine (Dog)Canine Dermal Fibroblast(SV40T OE)Normal
EBA02Canine Kidney Cell
EBB01Canine Bone OsteosarcomaBone osteosarcoma
EBB02Canine Kidney sarcomaKidney sarcoma
EBB03Canine Breast carcinomaBreast cancer / Carcinoma
EBC01Canine Bone Marrow Mesenchymal Stem CellStem Cell
ECA01Feline (Cat)Feline Lung CellNormal
ECA02Feline Kidney Cortex Cell
EDA01MouseMouse Brain NeuroblastNormal
EDA02Mouse Embryo Fibroblast(NIH/Swiss)
EDA03Mouse Myoblast
EDA04Mouse Embryo Fibroblast(C57BL/6)
EDB01Mouse Liver HepatomaLiver cancer / Hepatoma
EDB02Mouse Leukemia Spleen LymphoblastLeukemia
EDC01Mouse Embryonic Stem CellStem Cell
EEA01RatRat Skin Fibroblast ExosomeNormal
EFA01MonkeyMonkey Kidney epithelial cellNormal
EHA01Bovine (Cow)Bovine Dermal Fibroblast ExosomeNormal

Other Exosomes Related Product

Exosome Membrane Permeabilization

EV-Perm™ Permeabilization Pretreatment Kit for Exosome Membrane

用於增強了外泌體膜的滲透性，使抗體能夠穿透並檢測外泌體內的蛋白。

 原廠產品資訊

品名貨號
EV-Perm™ Permeabilization Pretreatment Kit for Exosome Membrane
294-85701

Exosome Blocking

EV-Save™ Extravellular Vesicle Blocking Reagent

用於保護外泌體免受冷凍破壞，並抑制其吸附在試管等實驗室器具皿上。

 原廠產品資訊

品名貨號package
EV-Save™ Extracellular Vesicle Blocking Reagent
058-09261 1 ml
EV-Save™ Extracellular Vesicle Blocking Reagent for in vivo050-09461

Exosome Tracking/ Staining Kit

ExoSparkler Exosome Lableing Kit

 產品資訊

品名廠牌貨號應用
ExoSparkler Exosome Membrane Labeling Kit
Dojindo EX01 EX02 EX03 -Green -Red -Deep Red
ExoSparkler Exosome Protein Labeling Kit
Dojindo EX04 EX05 EX06 -Green
-Red
-Deep Red

Human Exosomal Antibody Markers

Exosome Marker Antibody

 product list產品資訊

Exosome Marker Antibody

 產品資訊

Exosomal miRNA Extraction

miRQuick™ (Exosomal miRNA Extraction Kit)

產品資訊

TecnologyCharge-based precipitation & Silica membrane
FomatMini Prep
Package Unit10 Prep(s), 50 Prep(s)
HandlingCentrifugation
Sample materialPlasma, Serum, Urine, Saliva, Cell culture media, Other body fluids
Sample amount50 ~ 20000㎕
Elution volume20 ~ 100㎕
Preparation time45-60min
Typical downstream applicationArray technology, Northern blotting, Primer extension, qRT-PCR, RNA-Seq

Co-culture system

ICCP水平式細胞共同培養系統(co-culture system)

 產品資訊

Exosome free FBS

EXOSOME FREE FETAL BOVINE SERUM
去外泌體胎牛血清 

 產品資訊

貨號: FBS002-X 包裝: 50, 250 mL

  • 外泌體去除率: ≥ 95%
  • 內毒素 (Endotoxin) 含量 ≤10 EU/mL
  • 血紅蛋白(Hemoglobin)含量 ≤20 mg/dL
  • 三道0.1 μm Filter無菌過濾
  • 不含病毒和支原體 (Mycoplasma)
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