NEW DNA-In® CRISPR Transfection
Specifically formulated for CRISPR/Cas9 transfection reagent
DNA-In® CRISPR transfection reagent is optimized for large plasmid delivery of CRISPR/Cas9 vectors into a range of cell types. This reagent is especially well-suited for hard-to-transfect primary cells. Below, a Cas9-GFP plasmid was delivered into primary fibroblasts, muscles cells, keratinocytes and others primary cells and commonly used cell types with high efficiency and low toxicity.
Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays. Dojindo’s highly water-soluble tetrazolium salt, WST-8, is reduced by dehydrogenase activities in cells to give a yellow-color formazan dye, which is soluble in the tissue culture media. The amount of the formazan dye, generated by the activities of dehydrogenases in cells, is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than the other tetrazolium salts such as MTT, XTT, MTS or WST-1.
Phos-tag™ is a functional molecule that binds specifically phosphorylated ions.
It is applicable for the specific separation of phosphorylated proteins (Phos-tag™ Acrylamide) as well as for the detection using western blot (Phos-tag™ Biotin), enrichment, separation and purification (Phos-tag™ Agarose), and MALDI-TOF/MS (Phos-tag™ Mass Analytical Kit).
Methods for determining the phosphorylation status of proteins (i.e., phosphoproteomics) are thus very important with respect to the evaluation of diverse biological and pathological processes.